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. 2019 Aug 1;10:3460. doi: 10.1038/s41467-019-11494-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Analysis of killing activity by synthesized 3825 peptide. a Growth inhibition assays by the 3825 peptide (bacteroidetocin A) when 1 µg is applied in a 5 µl spot on top of an agar overlay containing the indicated strains. b Growth inhibition assays in broth culture when 2 µg of bacteroidetocin A (red) or water control (blue) is added to the indicated strain. All data represent the average of biological triplicates, ±SEM. c Colony forming unit (cfu/ml) quantification of viable bacteria following four hours in growth medium with bacteroidetocin A (red) compared to the starting cfu/ml (gray), or after four hours growth with added water (blue). Biological triplicates were performed for the growth assay with added bacteroidetocin A. Error bars represent standard error of the mean. d Agar overlay assays demonstrating the amount of bacteroidetocin A necessary to inhibit the growth of the overlay strains. e Titration of 3825 peptide in broth to determine the minimal inhibitory concentration for growth inhibition of B. thetaiotaomicron VPI 5482 and P. goldsteinii CL02. Source data are provided as a Source Data File