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. 2019 Aug 1;9:11197. doi: 10.1038/s41598-019-47693-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Differential energy transition intensity curves for oxFA and unoxidized FA species. The curves were fit with a sigmoidal non-linear regression. The intensities are displayed relative to the highest intensity transition. The collision energy (CE) yielding the highest intensity transition was designated as the quantitative transition (solid line). The CE yielding an intensity of roughly 50% of maximal was designated at the qualitative transition (dashed line). The qualifier ion ratio (QIR) was calculated based on the intensities of these two transitions. Differential energy curves were performed in triplicate with data expressed as mean ± S.E.M.