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. 2019 Aug 1;9:11197. doi: 10.1038/s41598-019-47693-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Method validation with a complex mixture of species and regioisomers. (a) Full MS scan of oxFA mixtures produced by auto-oxidation of LA and AA for specified time period. Both unoxidized and auto-oxidized spectra were measured under the optimized conditions for the unoxidized species. Peak height is displayed relative to the highest intensity peak in the spectra. (b) Chromatograms of 100 μM auto-oxidized LA and AA for specified amount of time. Zoomed individual analyte chromatograms depict both the quantitative (solid line) and qualitative transitions (dashed line). Peak height is displayed relative to the highest intensity peak in each chromatogram.