Table 2.

Method validation parameters.

Fatty Acid Species	Intra-assay Precision (%)	Interassay Precision (%)	Accuracy (%)	LOQ (pmol)	Retention Time (min)	Relative Retention Time
LA	0.8	5.2	96–109	0.01	10.89 ± 0.019	0.95
AA	1.7	6.9	88–104	0.01	10.85 ± 0.022	0.95
HPODE	3.7	6.0	82–101	0.1	10.50 ± 0.011	0.90
HODE	1.2	8.0	94–104	0.1	10.31 ± 0.031	0.90
KODE	6.5	10.0	98–110	0.1	10.27 ± 0.028	0.90
HPETE	2.5	8.2	81–100	0.1	10.37 ± 0.023	0.91
HETE	2.0	4.3	91–111	0.1	10.47 ± 0.027	0.92
KETE	6.2	10.8	98–113	0.1	10.42 ± 0.025	0.91
C17:0	1.7	8.0	95–102	10	11.11 ± 0.055	1.00

The intra-assay precision was determined by measuring five concentrations three times each on the same day. The interassay precision was determined by measuring five concentrations on five days. Precision values are expressed as the percent value of the standard deviation compared to the mean value. Accuracy was determined by measuring three different, known concentrations five times. Accuracy values are expressed as the percent value of the calculated concentration compared to the known concentration. The lower limit of quantification (LOQ) was determined to be the concentration at which the qualitative transition had a signal-to-noise ratio of greater than 3:1. The variation in retention time was determined by comparing retention times on five days and is expressed as the average retention time and standard deviation. The relative retention time compared to the internal standard C17:0 was calculated for each species. This value is constant for all three described HPLC methods.