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. 2019 Aug 1;4:33. doi: 10.1038/s41541-019-0129-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — CD4 and CD8 T-cell localization in the vagina of guinea pigs that received trivalent vaccine Prime with or without Ivag/topical imiquimod Pull. Vaginas from four groups were collected at the termination of the study (day 70) and frozen in OCT. Frozen sections were stained with antibody to CD8 and CD4 and analyzed by fluorescence microscopy using the ×10 objective lens. Scale represents 100 μm. a CD8 staining b CD4 staining. The L notation marks the lumen of the vaginal cavity