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. 2019 Aug 1;9:11213. doi: 10.1038/s41598-019-47229-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunocytochemistry and flow cytometry analysis of SNAP25 and TUBIII, and transcript levels of Map2 and Snap25. (a) SNAP25⁺ ADSC-CCs on days 3, 7, and 14, indicating the capacity of BRC to induce the differentiation of ADSC when in co-culture. (b) TUBIII⁺ ADSC-CCs on days 3, 7, and 14, indicating the capacity of BRC to induce the differentiation of ADSC when in co-culture. (c) Graphs show the percentage of positive cells for TUBIII (left) and for SNAP25 (right) on days 3, 7 and 14. (d) Graph on the left shows transcript levels of Map2, demonstrating an increase on day 7 compared to day 3 and graph on the right shows transcript levels of Snap25, demonstrating an increase on day 7 compared to day 3. TUBIII- beta tubulin III; SNAP25- synaptosomal-associated protein 25. Map2- microtubule-associated protein 2. Different letters indicate p < 0.05. Graph bars correspond to standard error. Bar = 100 µm.