Fig. 4.

The NS3 degraders inhibit telaprevir-resistant HCV. Huh7.5 cells were infected with wildtype HCV-Jc1 or the indicated telaprevir-resistant viruses at a MOI of 0.1. The infected cells were treated from 24 to 48 h post-infection with a range of small molecule concentrations (indicated in nM). a HCV NS3 and GAPDH abundance was evaluated by Western blot. Source data are provided as a Source Data file. One representative experiment is shown from n = 4. NS3 abundance was normalized to the loading control (GAPDH) and is presented as a percentage of the DMSO-treated control samples. Values represent the means of n = 4 independent experiments. b The amount of infectious virus released to the supernatants at 48 h post-infection was measured using a 50% tissue infectious dose (TCID₅₀) assay. The concentration of compound that led to a 50% reduction in viral titers (IC₅₀) was determined by nonlinear regression. Data are presented as means normalized to DMSO ± standard error of n = 4 technical replicates. One representative experiment is shown, with IC₅₀ values averaged from n ≥ 2 independent experiments