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. 2019 Aug 1;9:11177. doi: 10.1038/s41598-019-47581-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of extracellular vesicles from HCT116 and SW480 cell lines under rapamycin and cyclosporine A. Characterization of EVs released by HCT116 and SW480 CRC cell lines. (a,c) NTA measurement shows the concentration and size distribution of (a) HCT116EVs and (c) SW480EVs. (b,d) Images from cryo-electron microscopy of purified (b) HCT116EVs and (d) SW480EVs (scale bars 0.2 and 0.1 μm. (e) Bead-based flow cytometry analysis of HCT116EVs and SW480EVs stained with EV markers: CD9, CD81, and CD63. (f,g) EV production in Untr, RAPA, and CsA treatment was calculated as EVs/ml per total cells in HCT116 and SW480. Data are expressed as the mean ± SD. (n = 4). *P < 0.05 versus Untr (untreated) by Student’s t-test.