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. 2019 Aug 1;9:11177. doi: 10.1038/s41598-019-47581-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Validation of differentially expressed miRNAs in HCT116 and SW480 cell lines and their EVs with qRT-PCR. (a) miR-6127, miR-6746-5p, and miR-6787 relative expression in HCT116EVs and SW480EVs was normalized to EV production in Untr, RAPA, and CsA treatment. Data are expressed as the mean ± SD. (n = 3). *P < 0.05 versus Untr (untreated) and ≠P < 0.05 versus CsA treatment. (b) miR-6127, miR-6746-5p, and miR-6787 relative expression in HCT116 and SW480 in Untr, RAPA, and CsA treatment. Data are expressed as the mean ± SD. (n = 6). *P < 0.05 versus Untr (untreated) and ≠ P < 0.05 versus CsA by Student’s t-test.