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. 2019 Aug 1;9:11191. doi: 10.1038/s41598-019-47665-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The transcripts of snai2 are downregulated during CNC migration but upregulated again as CNC differentiates. In situ hybridization was performed for snai2 with wild-type X. tropicalis embryos at the indicated stages. The expression of snai2 in the CNC decreases from stage ~22 to ~31 (A–E), but elevates again in the CNC cells that form condensing mesenchyme in the pharyngeal arches (pa; F) and persists in the differentiating head cartilage structures (G,H). All embryos are shown with anterior to the left. (A–G) side view; (H) dorsal view. Insets in (G,H) are ventral view of head cartilage dissected from tadpoles after in situ hybridization, with anterior at the top. ln, lens; so, somites.