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. 2019 Aug 1;9:11191. doi: 10.1038/s41598-019-47665-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Wnt signaling activity in the CNC lineage. Heterozygous transgenic Wnt reporter embryos were imaged at the indicated stages. Expression of eGFP is detectable in the pre-migratory (A,B), migrating (C) and differentiating (D–F’) CNC. (A,B) dorsal view with anterior at the top; green fluorescence and bright-field images are merged to show the relative positions of CNC in the whole embryo. (C–E) side view with anterior to the left (C) or right (D,E). (F,F’) dorsal and ventral views (with anterior at the top), respectively, of the same tadpole. br, brain; ln, lens; bc, branchial cartilage; nV, trigeminal nerve; oe, olfactory epithelium; pa, pharyngeal arches.