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(A) TUNEL and (B) Annexin V assays. Percentage rise in apoptosis for the variants was normalized to that of the healthy controls. When compared to the healthy control, posttreatment apoptosis was lower for all the BARD1 variants, except BARD1 c.73G>C. (*) P < 0.05, (**) P < 0.005. Cells containing TP53 c.817C>T were included as positive control of impaired apoptosis. An independent t-test was used to compare the results between the variants and healthy controls.
