TABLE 1.
List of genes quantified by PCR
| Gene | Encoding Protein | Sequence |
| Abhd5 | 1-Acylglycerol-3-phosphate O-acyltransferase (also known as CGI-58) | Forward: 5′-TTGGGTTAAGTCTAGTGCAG |
| Reverse: 5′-TTTTTGAAAGCTGTCTCACC | ||
| Arsb | Arylsulfatase B | Forward: 5′-ACACGCCGAGGATTCGATAC |
| Reverse: 5′-CAAAGACTAATCGGCGCAC | ||
| Asah1 | N-Acylsphingosine amidohydrolase 1 | Forward: 5′-AGGACGTACTGAGACCCGAA |
| Reverse: 5′-GACAAGAGGCCTTGAGCCTT | ||
| Cd36 | Cluster of differentiation 36 (also known as fatty acid translocase, FAT/CD36) | Forward: 5′-CATTTGCAGGTCTATCTACG |
| Reverse: 5′-CAATGTCTAGCACACCATAAG | ||
| Cerk | Ceramide kinase | Forward: 5′-TGGTCTTGCTGGCTTCAAGATT |
| Reverse: 5′-ATGAGGGGAGGCCATAGTCTG | ||
| Cpt-1b | Carnitine palmitoyltransferase 1B | Forward 5′-ACTAACTATGTGAGTGACTGG |
| Reverse: 5′-TGGCATAATAGTTGCTGTTC | ||
| Dgat2 | Acyl-CoA:diacylglycerol acyltransferase 2 | Forward 5′-GTGGCAATGCTATCATCATC |
| Reverse: 5′-CTGCTTGTATACCTCATTCTC | ||
| Glb1 | Galactosidase, β1 | Forward 5′-TTCCGGATACCCCGCTTCTA |
| Reverse: 5′-GAAGTTCAAGGGCACGTACA | ||
| Gpam | Glycerol-3-phosphate acyltransferase 1, mitochondrial (also known as GPAT1) | Forward 5′-CATTCAGATTCACAAGGGTC |
| Reverse: 5′-GTGAATCAAGGTACTGAAGAC | ||
| Hexb | Hexosaminidase B | Forward 5′-GGTTGGTCCAAAGACTGCCT |
| Reverse: 5′-GACACTAGCGACACCAGCG | ||
| Lipe | Hormone-sensitive lipase | Forward 5′-AACTCCTTCCTGCAACTAAG |
| Reverse: 5′-CTTCTTCAAGGTATCTGTGC | ||
| Pdk4 | Pyruvate dehydrogenase kinase isozyme 4, mitochondrial | Forward 5′-ACAATCAAGATTTCTGACCG |
| Reverse: 5′-TCTCCTTGAAAATACTTGGC | ||
| Pisd | Phosphatidylserine decarboxylase | Forward 5′-TGTAACACATGCCAACAAGG |
| Reverse: 5′-ATCTTATAAGGTCAGCCCCG | ||
| Plin2 | Perilipin 2 (also known as adipose differentiation-related protein, ADRP) | Forward 5′-ATAAGCTCTATGTCTCGTGG |
| Reverse: 5′-GCCTGATCTTGAATGTTCTG | ||
| Plin5 | Perilipin 5 (also known as lipid storage droplet protein 5, LSDP5) | Forward 5′-TGTAGTGTGACTACCTGTG |
| Reverse: 5′-ATGTCACCACCATGTCTG | ||
| Pnpla2 | Patatin-like phospholipase domain containing 2 (also known as adipose ATGL) | Forward 5′-CAACCTTCGCAATCTCTAC |
| Reverse: 5′-TTCAGTAGGCCATTCCTC | ||
| Ppargc1a | PPARG coactivator 1α | Forward 5′-TCCTCTTCAAGATCCTGTTAC |
| Reverse: 5′-CACATACAAGGGAGAATTGC | ||
| Ptdss1 | Phosphatidylserine synthase 1 | Forward 5′-ATTCTCCCTGTCTACCCTGG |
| Reverse: 5′-TGCATACACTTCCATTCCCT | ||
| Sgpl1 | Sphingosine phosphate lyase 1 | Forward 5′-ATGTGGATGCTTGTCTGGGG |
| Reverse: 5′-GTCACACCTTTCACCCGGAA | ||
| Acta1 | Skeletal actin | Forward 5′-TATTCCTTCGTGACCACAGCT |
| Reverse: 5′-CGCGAACGCAGACGCGAGTG |