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A: Time-dependent release of FAEs from their NAPE precursors in pooled plasma during hydrolysis by guanidine hydroxide (0.5 M in methanol) at 60°C (n = 3). B: Time-dependent release of glycerol esters during incubation of pooled plasma at pH 4.7 and 25°C (n = 3). C: Incubation of pooled plasma at pH 4.7 and 25°C in presence of 5 µM Orlistat (n = 3).
