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Position specificity of FADS2 activity and absence of Δ8-desaturase activity. A, B: GC-MS of FAMEs of total lipids of mock-transfected (A) and fads2-transfected (B) HEK293 cells incubated with [D6]ω6-sciadonic acid. C, D: WT (C) and fads2−/− (D) MEFs incubated with [1-13C]ω6-linoleic acid and [D6]ω6-sciadonic acid.
