Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Aug 1;33(15-16):960–982. doi: 10.1101/gad.325142.119

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 Core and Adelman; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

PMC Copyright notice

Figure 5. — Distinct locations and mechanisms underlie regulated Pol II pausing and nucleosome-dependent pausing. (A) Pausing regulated by DSIF and NELF occurs with a predominant peak located 30-nt downstream from the TSS (range = 25–50 nt). This pausing can be long-lived and is clearly modulated to tune gene expression. Release of DSIF/NELF-mediated pausing is triggered by recruitment of P-TEFb to the gene promoter. (B) Upon pause release, Pol II soon encounters the first downstream nucleosome, called the +1 nucleosome. (C) The presence of a nucleosome can transiently halt RNA synthesis and induce Pol II arrest. Association of elongation factors and chromatin modifying complexes greatly facilitate Pol II movement through nucleosomes in vivo, making this pause short lived.