Figure 1.

High throughput analysis of acoustic startle responses in zebrafish larvae. A, Behavioral tracking of multiple larvae. The positions of 18 zebrafish larvae (6 dpf) are shown in red tracks over 120 frames in response to an acoustic/vibrational stimulus (left). The vertical series (right) shows the startle response of a single larva, with the bar along the head segment indicating the orientation of the fish. B, Analysis of head orientation permits automated identification and measurement of responses. Response latency, C1 angle, and C1 duration are quantified as indicated.