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. 2007 Jun 6;27(23):6115–6127. doi: 10.1523/JNEUROSCI.0180-07.2007

Figure 2.

Figure 2.

NFI regulates process formation and migration. A, The NFI dominant repressor impairs neurite outgrowth from CGN reaggregates. CGNs were infected with lentivirus expressing HA-tagged NFI/EnR or EnR during cell reaggregation and examined 20 h after plating for neurite extension using β-III tubulin immunostaining. B, NFI is required for migration of CGNs from reaggregates. Top, Reaggregates were stained for HA to identify transduced CGN nuclei. Bottom, The distribution of HA+ neurons migrating from reaggregates. C, The NFI dominant repressor does not significantly affect neurite outgrowth in fully dissociated CGN cultures. Dissociated CGNs were infected with NFI/EnR or EnR virus on 0 DIV and stained for β-III tubulin on 2 DIV. D, NFI is required for dendrite formation in dissociated CGN cultures. CGNs were transduced with NFI/EnR or EnR lentiviruses and then stained for MAP2. Both dendrite length and number per cell were significantly reduced by the NFI dominant repressor relative to control. *p < 0.001, significantly different from control. Scale bars: A–C, 100 μm; D, 50 μm.