Table 1.

Summary of primary antibodies used for immunocytochemistry and Western blot in this study

Antibody	Type	Epitope	Dilution	Source	Specificity tests
mGluR1a	Rabbit polyclonal; affinity-purified	C-terminal residues 1180–1199 of rat mGluR1a	0.5 μg/ml (ICC), 0.04 μg/ml (WB)	Millipore	Testa et al. (1998) (PA-IHC); Ferraguti et al. (1998) (TC/KO-WB); Marino et al. (2001) (TC-WB); Kuwajima et al. (2004) (TC-WB, KO-IHC)
mGluR5	Rabbit polyclonal; affinity-purified	C-terminal residues 1152–1171 of rat mGluR5	0.86 μg/ml (ICC), 0.17 μg/ml (WB)	Millipore	Marino et al. (2001) (TC-WB); Kuwajima et al. (2004) (TC-WB, KO-IHC)
Homer1b/c	Rabbit polyclonal; preabsorbed with H2 and H3, then affinity-purified	Residues 152–366 of mouse H1c	1:5,000 (ICC)	Shiraishi et al. (2003)	Shiraichi et al. (2003) (TC-WB); also see Fig. 3 for KO-IHC
Homer2	Rabbit polyclonal; preabsorbed with H1 and H3, then affinity-purified	Residues 142–343 of mouse H2a	1:5,000 (ICC)	Shiraishi et al. (2003)	Shiraichi et al. (2003) (TC-WB); also see Fig. 3 for KO-IHC
Homer3	Rabbit polyclonal; preabsorbed with H1 and H2, then affinity-purified	Residues 131–358 of mouse H3	1:5,000 (ICC)	Shiraishi et al. (2003)	Shiraichi et al. (2003) (TC-WB)
Homer	Rabbit polyclonal	Residues 13–354 of human Homer1b	1:100 (WB)	Santa Cruz Biotechnology	Santa Cruz Biotechnology (WB)
DAT	Rat monoclonal	N terminus of human DAT fused to Glutathione S-transferase	1:500 (ICC)	Millipore	Miller et al. (1997) (TC-WB)
TH	Rabbit polyclonal; affinity-purified	Rat TH from pheochromocytoma denatured by SDS	1:5,000 (WB)	Millipore	Millipore (WB)
Actin	Goat polyclonal; affinity-purified	C terminus of human actin	1:500 (WB)	Santa Cruz Biotechnology	Santa Cruz Biotechnology (WB)

ICC, Immunocytochemistry; IHC, immunohistochemistry; KO, knock-out mouse tissue; PA, preadsorption; TC, transfected cells; WB, Western immunoblots.