Table 1.
Bicuculline (40 μm, 12 h) produced no change in NAC1, 20S, 19S, Cul3, or PSD95 in whole-cell lysates of cortical neurons infected with AAV-NAC1 or AAV-GFP
| Treatment | PSD Proteins |
||||
|---|---|---|---|---|---|
| 19S | 20S | Cul3 | PSD-95 | HA-NAC1 | |
| AAV-GFP | |||||
| Control | 100 ± 5.6 | 100 ± 3.7 | 100 ± 13.3 | 100 ± 7.7 | NA |
| Bicuculline | 107.7 ± 4.7 | 106.7 ± 6.0 | 110.1 ± 11.2 | 98.0 ± 15.4 | NA |
| AAV-NAC1 | |||||
| Control | 100 ± 16.4 | 100 ± 8.2 | 100 ± 8.1 | 100 ± 4.0 | 100 ± 13.7 |
| Bicuculline | 108.1 ± 13.2 | 86.1 ± 5.0 | 100.7 ± 5.1 | 81.2 ± 13.6 | 110.2 ± 11.1 |
Cells were incubated in bicuculline for 12 h before harvesting. n = 5 for all groups, data are shown as the mean ± SEM percentage change from GFP- or NAC1-infected cells incubated with control buffer. NA, Not applicable because cells were not infected with HA-tagged NAC1.