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Photomicrographs of Kiss1 mRNA-containing neurons in the AVPV nucleus of representative testosterone-treated WT and GPR54 KO male mice. GPR54 KO males had significantly more Kiss1 neurons in the AVPV nucleus than WT males, as well as greater numbers of silver grains per Kiss1 cell (see Results for numerical values). White arrows denote sample Kiss1 neurons. 3V, Third ventricle; o.c., optic chiasm. Scale bar, 50 μm.
