Table 1.

Comparison of somatic and reproductive characteristics of gonad-intact WT and GPR54 KO male and female mice

	Males		Females
	Wild type	GPR54 KO	Wild type	GPR54 KO
PND1 BW (g)	1.53 ±0.05	1.57 ±0.05	1.45 ±0.04	1.48 ±0.03
PND1 AGD (mm)	1.2 ±0.05	1.3 ±0.06	0.9 ±0.06	1.0 ±0.04
PND1 AGD ratio (mm/g)	0.83 ±0.04	0.83 ±0.04	0.65 ±0.04	0.65 ±0.03
Day 21 BW (g)	10.2 ±0.4	9.8 ±0.4	10.0 ±0.5	9.4 ±0.4
Day 21 AGD (mm)	6.5 ±0.2	5.4 ±0.2*	3.2 ±0.1	3.0 ±0.1
Day 21 AGD ratio (mm/g)	0.65 ±0.02	0.56 ±0.02*	0.33 ±0.01	0.32 ±0.01
Adult BW (g)	28.0 ±1.0	25.1 ±1.0*	21.3 ±0.7	22.2 ±0.8
Ovaries (mg)			19.9 ±0.9	7.5 ±0.7*
Testes (mg)	243.6 ±9.7	9.0 ±1.7*
Seminal vesicles (mg)	272.2 ±22.6	33.2 ±4.7*
Penis (mg)	52.5 ±6.1	19.5 ±2.0*
Plasma LH (ng/ml)	1.19 ±0.61	0.05 ±0.1*

All measurements were taken in young adult mice (∼2.5–4 months old), except prepubertal body weight (BW) and anogenital distance (AGD), which were measured at PND1 and 3 weeks of age. GPR54 KO males differed from WT males in every adult measure as well as day 21 AGD. GPR54 KO females did not differ from WT females on any measure except adult ovarian weight. Animal numbers were 10 animals per group (testes, seminal vesicles, penis, LH), 10–13 animals/group (adult BW), and 15–26 animals per group (prepubertal BW, AGD). Asterisks indicate significant difference from WT animals of same sex.