Table 2.

Male sexual behavior of gonad-intact and T-treated WT and GPR54 KO mice

	% Males mounting and thrusting	% Malesintromitting	% Males ejaculating	Mean latency to begin mounting (min)	Mean number of thrusting bouts	Mean number of intromission bouts
Gonad-intact
Wild-type	80	70	40	10.7 ± 1.5	6.1 ± 1.2	9.9 ± 1.7
GPR54 KO	0*	0*	0*	na	na	na
T-treated
Wild-type	71	71	64	7.1 ± 1.8	4.9 ± 1.0	9.7 ± 2.7
GPR54 KO	83	67	17*	8.8 ± 1.6	15.7 ± 3.4*	9.3 ± 2.2

All male mice were paired with a hormone-primed sexually receptive female for 45 min. Gonad-intact GPR54 KO males did not display any sexual behavior. In contrast, castrated T-treated GPR54 KO males displayed mounting, thrusting, intromissions, and in the case of two animals, an ejaculation. Animal numbers were 10 animals per group (gonad-intact) and 12–14 animals per group (T-treated). *p < 0.05, significantly different from similarly treated WT males.na, Not applicable because gonad-intact KO males did not display any sexual behavior.