Figure 5.

Expression of NQO-1 and OCAM are unaltered in robo-2^−/− and slit-1^−/−;slit-3^−/− mice. A–N, In situ hybridization of coronal sections of olfactory epithelia from P0 wild-type (A, D, G, I, L), slit-1^−/−;slit-3^−/− (B, E, H, J, M), and robo-2^−/− (C, F, K, N) mice with cRNA probes specific for NQO-1 (A–C), OCAM (D–F), robo-2 (G, H), robo-1 (I–K), and rig-1 (L–N). robo-2 is expressed in a gradient in the OE with high levels of expression in the dorsomedial region of the OE and low levels in the ventrolateral region in wild-type (G) and slit-1^−/−;slit-3^−/− (H) mice. NQO-1 is expressed in the dorsomedial region, whereas OCAM is expressed in the ventrolateral region of the olfactory epithelium in wild-type (A, D), slit-1^−/−;slit-3^−/− (B, E), and robo-2^−/− (C, F) mice. Both robo-1 and rig-1 are not expressed in the olfactory epithelium in wild-type (I, L), slit-1^−/−;slit-3^−/− (J, M), and robo-2^−/− (K, N) mice. n = 5 robo-2^−/− and n = 5 slit-1^−/−;slit-3^−/−. O, P, In situ hybridization of parasagittal sections of olfactory bulbs from E16 slit-1^−/− embryos with cRNA probes specific for slit-3 (O) and slit-2 (P). Whereas slit-3 is expressed in the ventral region of the olfactory bulb, slit-2 is not expressed in the olfactory bulb of slit-1^−/− embryos. n = 3 slit-1^−/−. D, Dorsal; V, ventral; L, lateral; M, medial; R, rostral; C, caudal. Scale bars, 250 μm.