Figure 5.

Glutamine enhances quantal amplitude in dissociated hippocampal culture with the same time course as in hippocampal slices. A, Cumulative distribution function of amplitudes is not different between 4 mm glutamine (Gln)-incubated (n = 18) and control cultures (n = 20; p > 0.05, Kolmogorov–Smirnov test). Sample recordings from control (top) and glutamine-incubated (bottom) cells. Calibration: 10 pA, 100 ms. B, Cumulative distribution function of amplitudes is different between 4 mm glutamine-incubated (n = 15) and control cultures (n = 14; p < 0.05, Kolmogorov–Smirnov test). Sample recordings from control (top) and glutamine-incubated (bottom) cells. Calibration: 10 pA, 100 ms. C, Current evoked by sucrose (500 mm) does not differ between cultures incubated in 4 mm glutamine (<4 h, n = 19; >4 h, n = 16) relative to control coverslips (<4 h, n = 16; >4 h, n = 16; p > 0.05). Inset, Representative traces show sucrose-evoked currents. Black bars indicate application of 500 mm sucrose. Calibration: 400 pA, 1 s.