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. 2007 May 30;27(22):6068–6078. doi: 10.1523/JNEUROSCI.1442-07.2007

Figure 2.

Figure 2.

NGF/Sema3A treatment restored lamina-specific projections of CGRP+ axons after dorsal root injury. To quantify the laminar distribution of regenerated axons, a mask of six consecutive boxes were drawn on the representative image of normal spinal cords, outlining spinal cord laminas I–VI (A). This was used as a template and applied to the appropriate position of the spinal cord. CGRP axon-occupying area was quantified in each lamina. B, In normal spinal cord, CGRP+ axons are mostly located in superficial laminas I and II. Dorsal root injury almost completely abolished CGRP fibers in all laminas. NGF treatment induced robust regeneration of axons in all laminas, which is significantly different from the normal distribution. In contrast, NGF/Sema3A-induced regeneration is more selectively localized to superficial laminas I and II, which closely patterned the normal distribution. Scale bar, 0.5 mm. Values represent mean ± SD; n = 3–6. *p < 0.05, **p < 0.01 compared with normal spinal cord; ## p < 0.01 compared with NGF+GFP group, analyzed by two-way ANOVA.