Table 3.
Microarray analysis of hippocampal tissue of mice that consumed (−)epicatechin
| Gene | Description | t statistic ESvCS | t statistic ERvCR | Affymetrix probe set identifier |
|---|---|---|---|---|
| A. (−)Epicatechin increases gene expression | ||||
| Nfatc1 | Nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1 | 34 | 1.19 | 1425761_a_at |
| Sart2 | Squamous cell carcinoma antigen recognized by T-cell 2 | 19 | 0.67 | 1455795_at |
| Notch1 | Notch gene homolog 1 (Drosophila) | 11.62 | −0.42 | 1418633_at |
| Ptpn6 | Protein tyrosine phosphatase, nonreceptor type 6 | 11 | −0.3 | 1460188_at |
| Parvg | Parvin, γ | 10.91 | 1.21 | 1416876_at |
| Mfap2 | Microfibrillar-associated protein 2 | 10.51 | −1.95 | 1417359_at |
| Zfp553 | Zinc finger protein 553 | 10.42 | 0.03 | 1426564_at |
| Cdc20 | Cell division cycle 20 homolog (Saccharomyces cerevisiae) | 9.05 | 1.77 | 1416664_at |
| Smad5 | MAD homolog 5 (Drosophila) | 8.75 | −0.47 | 1451873_a_at |
| Pomt2 | Protein-O-mannosyltransferase 2 | 8.67 | −0.55 | 1433644_at |
| B. (−)Epicatechin decreases gene expression | ||||
| Clca5 | Chloride channel calcium activated 5 | −23 | 0.27 | 1438109_at |
| Exosc2 | Exosome component 2 | −12.25 | −1.54 | 1426630_at |
| Cd52 | CD52 antigen | −10.25 | −3.15 | 1460218_at |
| Scx | Scleraxis | −9.5 | −1.4 | 1456291_x_at |
| Tm4sf5 | Transmembrane 4 superfamily member 5 | −8.85 | 0.77 | 1424445_at |
| Svs6 | Seminal vesicle secretion 6 | −8.49 | 0.59 | 1418548_at |
| Trim45 | Tripartite motif-containing 45 | −8 | 0.71 | 1441412_s_at |
| Lpl | Lipoprotein lipase | −7.86 | 0.21 | 1415904_at |
| Rpl35 | Ribosomal protein L35 | −7.82 | −0.02 | 1434231_x_at |
| Hes3 | Hairy and enhancer of split 3 (Drosophila) | −7.37 | 0.19 | 1422409_at |
| C. (−)Epicatechin and running increase gene expression | ||||
| Amh | Anti-Mullerian hormone | −2.25 | 10.84 | 1450573_at |
| Kif17 | Kinesin family member 17 | −0.75 | 10.09 | 1422762_at |
| Snap25 | Synaptosomal-associated protein 25 | 0.55 | 9.14 | 1416828_at |
| AI836003 | Expressed sequence AI836003 | 0.28 | 9.09 | 1435512_at |
| Zfp535 | Zinc finger protein 535 | 0.15 | 8.95 | 1418033_s_at |
| Hist1h4j | Histone cluster 1, H4c | 1.94 | 8.76 | 1431658_at |
| Mapk12 | Mitogen-activated protein kinase 12 | 0.3 | 7.67 | 1449283_a_at |
| Ogg1 | 8-Oxoguanine DNA-glycosylase 1 | 1.27 | 7.55 | 1430078_a_at |
| Ap3s2 | Adaptor-related protein complex 3, σ 2 subunit | 1.43 | 7.43 | 1441993_at |
| Impdh1 | Inosine 5′-phosphate dehydrogenase 1 | −0.05 | 7.41 | 1423239_at |
| Hey1 | Hairy enhancer of split with YRPW motif | −0.72 | 7.16 | 1419303_at |
| D. (−)Epicatechin and running decrease gene expression | ||||
| Dyrk2 | Dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 2 | 1.78 | −12.5 | 1456502_at |
| Tnf | Tumor necrosis factor | −0.77 | −10.06 | 1419607_at |
| Tmem102 | Transmembrane protein 102 | 2.99 | −8.97 | 1453318_at |
| Rpusd1 | RNA pseudouridylate synthase domain containing 1 | −0.11 | −8.83 | 1453253_a_at |
| Nup43 | Nucleoporin 43 | 0.65 | −8.72 | 1432188_s_at |
| Lypla3 | Lysophospholipase 3 | 0.41 | −8.49 | 1422341_s_at |
| St3gal1 | ST3β -galactoside α -2,3-sialyltransferase 1 | 1.58 | −8.49 | 1418946_at |
| Cyb5r2 | Cytochrome b5 reductase 2 | −0.83 | −8.18 | 1459448_at |
| Pitpnm1 | Phosphatidylinositol membrane-associated 1 | −0.06 | −7.9 | 1425760_a_at |
| Elovl3 | Elongation of very long chain fatty acids | 0.12 | −7.46 | 1420722_at |
Microarray analysis of hippocampal tissue in mice fed (−)epicatechin daily for 6 weeks with or without physical activity. The 10 annotated genes with highest t statistics that are either upregulated (A, C) or downregulated (B, D) by (−)epicatechin in sedentary (A, B) or running (C, D) mice are listed. ESvCS, ES versus CS; ERvCR, ER versus CR.