Figure 3.

PKC is not involved in histamine potentiation. A, Mean data of normalized exocytotic efficiency measured in the presence of histamine (100 μm; n = 19), calphostin C (Cal) (100 nm) plus histamine (100 μm; n = 6), and Bis (0.5–1 μm) plus histamine (n = 7). B, Inset, Overlaid sample traces of I _Ca before (ctrl) and 4 min into PMA (250 nm) application. Bar graph, Mean ± SEM percentage change in peak I _Ca by PMA (250 nm) in untreated (n = 6) and Bis (0.5–1 μm)-treated cells (n = 7). Note that the experiment represented in B was performed on the same cells represented in A and confirms the effectiveness of the Bis pretreatment. **p < 0.01.