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. 2007 Jan 3;27(1):15–26. doi: 10.1523/JNEUROSCI.3826-06.2006

Figure 1.

Figure 1.

Identification of Kinesin-1 as a novel DISC1-interacting molecule. A, B, Cytosolic extract from rat brain were used in affinity-column chromatography with MBP–DISC1. Aliquots from eluates were resolved by SDS-PAGE using 6% gel (A) to high-molecular-weight proteins and 12% gel (B) to low-molecular-weight proteins, followed by silver staining. Arrows indicate the DISC1-interacting proteins. Bound proteins were analyzed by mass spectral analyses. Asterisks indicate full-length or degradation product of MBP–DISC1. C, The eluates of DISC1 affinity-column chromatography were analyzed by immunoblotting with antibodies against kinesin family, including KIF5B, KLCs, KIF5A, KIF3B, and KIF2. D, The eluates of DISC1 affinity-column chromatography were analyzed by immunoblotting with antibodies against the NUDEL/LIS1/14-3-3ε/cytoplasmic dynein complex, including 14-3-3ε, NUDEL, LIS1, DIC, and CDHC, and RhoGDI as a negative control. Aliquots of original samples (2% Input) and eluates (10%) were subjected to SDS-PAGE.