Figure 4.
Transfection of RalA or RalB restores GTP-dependent exocytosis in the RalA/RalB double knock-down cells. A, Secretion of transfected NPY-hPLAP was stimulated with the indicated concentrations of GppNHp from wild-type PC12 cells (WT; gray diamonds) or RalA/RalB double knock-down (RalAKD8/RalBKD9) cells (DKD; black squares). The error bars indicate SEM (n=6). B, GppNHp-induced secretion of NPY-hPLAP from the double knock-down cells transfected with Myc-RalA (DKD plus Myc-RalA; black squares) or a control vector (DKD; gray diamonds). The transfected cells were permeabilized and stimulated with the indicated concen-trations of GppNHp. The error bars indicate SEM (n=8). C, GppNHp-induced secretion of NPY-hPLAP from the double knock-down cells transfected with pCMVmyc-rRalB(SNM) (DKD plus Myc-RalB; black squares) or a control vector (DKD; gray diamonds). The transfected cells were permeabilized and stimulated with the indicated concentrations of GppNHp. The error bars indicate SEM (n=6).