Figure 1.
LINGO-1 and NGF inhibit oligodendrocyte differentiation and myelination of TrkA positive DRG neurons. A, Anti-MBP immunostaining (red) of OPC/DRG cocultures after 14 d in culture in the presence of a control IgG, an anti-LINGO-1 antibody (1A7) or in the absence of NGF with the addition of the NGF scavenger TrkA-Fc. B, Anti-MBP immunostaining (red) of OPC/DRG cocultures after lentiviral transduction of DN- and FL-LINGO-1 in DRGs, OPCs, or both cell types. C, The number of myelinating MBP+ oligodendrocytes/field were quantified for the expression of FL-LINGO-1, DN-LINGO-1, and the control in each of the conditions. *p < 0.05; **p < 0.0001, unpaired t test. D, Western blot analysis for MAG, MBP, and β-actin, of OPC/DRG cocultures infected with FL and DN forms of LINGO-1, NgR, TROY/Taj, p75NTR, and TrkA after 14 d in culture. E, Electron micrographs of spinal cord sections were analyzed for extent of myelination in cross sections from a transgenic mouse overexpressing axonal LINGO-1 at postnatal day 8 and a wild-type littermate control. F, The number of myelinated axons in the spinal cords were counted and normalized per field. Error bars indicate SD. *p < 0.001, unpaired t test. Scale bars, 100 μm.