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. 2007 Apr 18;27(16):4273–4282. doi: 10.1523/JNEUROSCI.3477-06.2007

Figure 1.

Figure 1.

Disruption of Sef in SefKST223 gene trap mutants. a, SefKST223 mice contain a gene trap vector resulting in a fusion of β-geo to Sef protein at the position indicated (Leighton et al., 2001; Mitchell et al., 2001). Primers specific for Sef-β-geo and for the 3′ end of Sef are indicated by arrows. SS, Signal sequence; TM, transmembrane domain; IL 17, interleukin 17; SA, splice acceptor. b, X-gal staining of an E10 SefKST223 heterozygous embryo. Sef–β-geo activity is seen in many regions of FGF signaling, including the mid-hindbrain junction (asterisk) and the ventral half of the otic vesicle (arrow). c, Reverse transcription-PCR of E14.5 RNA collected from wild-type (WT), heterozygous (Het), and mutant (Mut) embryos with primers specific for Sef shows a moderate decrease in Sef expression levels in heterozygous embryos and a strong decrease in mutants. Conversely, Sef-β-geo is present in heterozygotes and mutants but not wild types.