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Molecular analysis of spinal cord development in Wnt1–Cre;Ext1flox/flox embryos. A–J, Transverse sections of E10.5 spinal cords of Wnt1–Cre;Ext1flox/flox and wild-type littermate embryos were analyzed by immunohistochemistry for Pax7 (A, B), En1 (C, D), and Nkx2.2 (E, F), and by in situ hybridization for Shh (G, H) and Ptc (I, J). fp, Floor plate; nc, notochord. Scale bar, 100 μm.
