Figure 6.

A, Transplanted E9.5-derived NSCs persist in the brain. E9.5 NSC-derived cells (green) remain in the developing brain, after transplant into the E9.5 telencephalon, and are widespread at P1. i, Whole mount of brain. ii, Regions derived from GFP(+) E9.5 definitive NSCs. iii, In contrast to ES-derived primitive NSCs, which were no longer present at this time point, cells remain in or next to ventricles in a scattered manner at P1, as shown in merge of bright field and GFP channel. Note the differentiated morphology of the transplanted cells. Ventricles are indicated by asterisks in sections. B, Transplanted adult-derived NSCs persist in the brain. Adult NSC-derived cells (green) are also present at P1, after transplant into the E9.5 telencephalon. i, Whole mount of brain. ii, Regions derived from GFP(+) adult definitive NSCs. iii, Similarly to the E9.5-derived definitive NSCs, the progeny of adult definitive NSCs remain in or next to ventricles and are scattered throughout these regions at P1, as shown in merge of bright field and GFP channel. Similar to the E9.5 NSC progeny, adult NSC progeny also display a differentiated morphology but in fewer cells than the E9.5-derived NSC descendants (see also Fig. 7A,B). Ventricles are indicated by asterisks in sections.