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. 2007 May 16;27(20):5271–5279. doi: 10.1523/JNEUROSCI.0883-07.2007

Figure 4.

Figure 4.

Genetic single-neuron labeling system and labeled neurons. A, Single-neuron labeling system in combination with Cre/loxP and Gal4/VP16 systems. B–K, Filled arrowheads indicate the soma of a labeled neuron. Open arrowheads indicate the final projection site of the labeled axon. B, J, K, An example of a brain with labeled neurons in the tectum at 7 dpf, shown in lateral view (B), dorsal view (J), and ventral view (K). C, Tectal neurons with dendrites labeled in different colors (brackets), at 7 dpf, shown in dorsal view. D–I, Several types of neuron labeled in this study shown in dorsal view (D, F, H) and lateral view (E, G, I): a neuron with wide-field radial dendrites and an ipsilaterally descending axon (D, E), a neuron with small-field horizontal dendrites and an ipsilaterally descending axon (F, G), an interneuron without a descending projection (F, small arrow), a neuron with an axon through the tectal commissure (H, arrowhead), and a neuron in the torus semicircularis (I). J, Open circle indicates the location of the soma. K, An overlaid image of labeled reticulospinal neurons indicates projection to r2 in the hindbrain. Scale bars: B, C, D, J, 50 μm.