Figure 7.

Polysomal incorporation of PSD-95 mRNA in the presence of cycloheximide, puromycin, and EDTA. Synaptoneurosomal extracts from wild-type mice were treated with 100 μg/ml cycloheximide (CHX), 1 mm puromycin, or 30 mm EDTA and the lysate was separated on 15–45% sucrose density gradient. a, A254 absorbance profiles from sucrose density gradients. b, qRT-PCR for PSD-95 mRNA in each fraction. c, Results pooled into three groups: mRNP/monosomes, light polysomes (L-poly), and heavy polysomes (H-poly). These results indicate that, after EDTA treatment, most of the mRNA is in mRNP fraction and cycloheximide results in mRNA predominantly in heavy polysomes, whereas puromycin treatment considerably decreases the mRNA in heavy polysome fraction.