Table 1.

Identification of three groups of PrP mutants based on their protection against Bax-mediated cell death in MCF-7 cells

	Protection against Bax		Amino acid		Secondary structure
	Val129	Met129	Polarity	Charge
Group I
WT	Yes	Yes	−	−	β-Sheet 1
A117V	Yes	Yes	−	−	TM
V203I	Yes	Yes	−	−	α-Helix C
Group II
V180I	Yes	Partial	−	−	α-Helix B
E200K	Yes	Partial	−	+	α-Helix C
T188A	Yes	Partial	+	−	α-Helix B
E196K	Yes	No	−	+	Between α-helix B/C
R208H	Yes	No	−	−	α-Helix C
Group III
E211Q	Partial	Partial	−	+	α-Helix C
D178N	Partial	No	−	+	α-Helix B
M232R	No	Partial	+	+	GPI anchor signal
V210I	No	No	−	−	α-Helix C
P238S	No	No	+	−	GPI anchor signal

Change in the polarity or the charge of the amino acid in the PrP mutation are indicated, using + if there is a change and − if there is no change. The location of the substituted amino acid in the secondary structure of PrP is provided. TM, Transmembrane domain.