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Patch-clamp analysis of desensitization of GluR3(Q)flop and GluR4(Q)flop. A, Glutamate-induced whole-cell responses of GluR3(Q)flop in the presence and absence of γ2 were recorded from HEK293 cells. The application of agonist (3 mm glutamate) is indicated by black bars. B, Quantification of the extent of desensitization [100% − (Isteady state/Ipeak) × 100%] of GluR3(Q)flop and GluR4(Q)flop in both the presence and absence of γ2. Data are shown ± SEM (n = 3–6).
