Figure 4.
Recovery of NMDA-evoked current after functional blockade of surface receptors is faster in cultured striatal MSNs from YAC72 versus wt mice. a, Sample trace for NMDA-induced current from whole-cell patch-clamp recording of cultured striatal MSNs at 9–10 d.i.v., before (Pre-Block) and after (Post-Block) blockade of surface NMDARs by coapplication of 1 mm NMDA and 5 μm MK-801. b, Representative recording from YAC72 MSN showing NMDA-evoked current recovery after MK-801 block; each current trace represents average of three consecutive responses to 500 ms pulses of 1 mm NMDA. c, Plot of recovery of NMDA-induced IAUC (integrated current, normalized to Cm) over time. Values represent mean ± SEM from n = 15, 10, 6 (wt) and 8, 6, 5 (YAC72) cells for 5, 10, and 15 min time points, respectively, after block by MK-801. *p < 0.05, **p < 0.01 determined by two-way ANOVA, Bonferroni's post hoc test.