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. 2007 Apr 4;27(14):3639–3649. doi: 10.1523/JNEUROSCI.4396-06.2007

Figure 5.

Figure 5.

Immunofluorescence double labeling of BACE1 together with Aβ, APP, or tau in 5XFAD and AD brains. Brain sections from 5XFAD or AD brains were costained with BACE-Cat1 and either antibodies against Aβ42 (A), Aβ40 (B), APP (C, top), or tau (C, bottom). A, 5XFAD (top) and AD (bottom) brain sections were double stained with BACE-Cat1 (red) and anti-Aβ42 C-terminal specific antibody (green). Note that BACE1 immunoreactivity surrounds the Aβ42 core of the plaques in both APP Tg and AD brains. B, AD brain section was double stained with BACE-Cat1 (red) and anti-Aβ40 C-terminal-specific antibody (green). Two representative amyloid plaques are shown. Note the high degree of colocalization between BACE1 and Aβ40 immunoreactivities. C, Top, AD brain section was double stained with BACE-Cat1 (red) and anti-APP C-terminal antibody (green). There is extensive colocalization of BACE1 and APP immunoreactivities within the deposit. C, Bottom, AD brain section was double stained with BACE-Cat1 (red) and anti-tau R1 antibody (green). Filamentous tau-positive structures tend to be concentrated around and partially overlap with the BACE1 deposit. Scale bar: A, Top left, 20 μm.