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. 2007 Apr 4;27(14):3639–3649. doi: 10.1523/JNEUROSCI.4396-06.2007

Figure 6.

Figure 6.

Immunofluorescence double labeling of BACE1 with neuronal and astrocytic markers in 5XFAD and AD brains. Brain sections from 5XFAD or AD brains were costained with BACE-Cat1 and either antibodies against NSE (A), synaptophysin (presynaptic marker) (B), MAP2 (postsynaptic marker) (C), β-III tubulin (neuronal marker) (D), or GFAP (astrocyte marker) (E). A, 5XFAD (top) and AD (bottom) brain sections were double stained with BACE-Cat1 (red) and anti-NSE antibody (green). Note that, within BACE1-positive deposits, BACE1 immunoreactivity colocalizes extensively (5XFAD) and moderately (AD) with NSE staining. B, 5XFAD (top) and AD (bottom) brain sections were double stained with BACE-Cat1 (red) and anti-synaptophysin antibody (green). Within deposits, BACE1 immunoreactivity colocalizes moderately well with synaptophysin staining in both 5XFAD and AD brains. C, 5XFAD brain sections were double stained with BACE-Cat1 (red) and anti-MAP2 antibody (green). BACE1 and MAP2 immunoreactivities do not colocalize to any significant extent. D, 5XFAD (top) and AD (bottom) brain sections were double stained with BACE-Cat1 (green) and anti-β-III tubulin antibody (red). BACE1 colocalization with β-III tubulin is present but relatively low within BACE1-positive deposits in 5XFAD and AD brains. E, 5XFAD (top) and AD (bottom) brain sections were double stained with BACE-Cat1 (red) and anti-GFAP antibody (green). Note that the overlap between BACE1 and GFAP immunoreactivities in 5XFAD and AD deposits is low. Scale bar: A, Top left, 20 μm.