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. 2007 Apr 4;27(14):3921–3932. doi: 10.1523/JNEUROSCI.4710-06.2007

Figure 4.

Figure 4.

Radial glia, basal lamina, and granule cell migration in Plexin-B2-deficient mice. A–H, Sagittal cerebellum sections immunostained for GFAP (A–D, G, H), laminin (C–F), and Pax6 (G, H). Normal radial glia palisade in adult (A), P15 (C), or P11 (G) Plexin-B2+/−. In contrast, the radial glia palisade is very perturbed in adult (B), P15 (D), and P11 (H) Plexin-B2−/−, although radial glia fibers still extend to the pial surface and form typical end feet (arrowheads in D). C, D, Laminin expression at the GFAP-positive radial glia end feet is similar in P15 Plexin-B2+/− (C) and Plexin-B2−/− (D) mice. E, F, In adult, Plexin-B2+/− (E) and Plexin-B2−/− (F) laminin is highly expressed in the basal lamina covering each cerebellar folia. The staining is similar in heterozygous and homozygous. G, H, At P11, radially migrating Pax6-expressing granule cells can be observed apposed to GFAP-positive radial glia fibers both in Plexin-B2+/− and in Plexin-B2−/− (arrowheads in G, H). Scale bars: A, B, 270 μm; C, D, 50 μm; E, F, 220 μm; G, H, 35 μm.