Figure 6.

Changes in tau species after suppression of transgene expression with doxycycline between 6.5 and 8 months. A, Transgene suppression with doxycycline led to decreased levels of ∼55 kDa tau, 64 kDa tau, and tau multimers (tau170 and tau140) in total extracts. B, Transgene suppression with doxycycline also led to decreased levels of tau species in the soluble (S1) fraction. C, Levels of sarkosyl-insoluble 64 kDa tau and tau multimers in the P3 fraction were variable and did not show a consistent decrease with transgene suppression. D, Quantification of changes of different tau species from experiment in Figure 6A–C. The values of tau species in animals not treated with doxycycline was set as 100% (n = 10 for animals without doxycycline; n = 11 for animals treated with doxycycline). ***p < 0.001; NS p > 0.05. Two-sample two-tailed t test was used.