Figure 7.
Expression of Cav1.3 mRNA in mature inner and outer hair cells using whole-mount in situ hybridization with organs of Corti of mouse and rat. A, C, Cav1.3 mRNA expression could be specifically detected in IHCs and in all three rows of OHCs in the mouse (A) and rat (C) organ of Corti (midbasal half-turn) at P19. B, D, No mRNA was detected when corresponding sense riboprobes were used in either mouse (B) or rat (D) whole-mount preparations. Large arrows indicate the row of inner hair cells, and small arrows indicate the rows of outer hair cells. Irregularities in IHC rows were a result of tissue shrinkage during the in situ hybridization procedure. Scale bar, 20 μm.