Figure 1.

Effect of anesthesia on tau phosphorylation. Proteins from mouse brain hemispheres (hippocampus plus cortex) were separated by SDS-PAGE and identified with the antibodies indicated on top of the boxes. For each antibody, two representative data of four for each condition (Ctl, control; Anes, anesthesia by chloral hydrate) are displayed. Each lane represents an individual mouse. The bands were quantified and the immunoreactivity during anesthesia expressed as a percentage of the control treatment (100%): A, 187 ± 7**; B, 42 ± 3*; C, 1471 ± 177***; D, 326 ± 12*; E, 15683 ± 8059*; F, 475 ± 77**; G, 176 ± 40*; H, 230 ± 23***; I, 116 ± 2. Data are means ± SD. *, **, and *** indicate significant differences with p < 0.05, 0.01, and 0.001, respectively.