Figure 5.

A, Vital staining with isolectin B4 (IB4; green) of dissociated DRG neurons from stages E18.5 and P14, showing preferential staining of small-diameter neurons and lack of staining in large-diameter neurons (asterisks). Scale bar, 50 μm. B, Percentage of IB4-binding neurons at different developmental stages. C, Pie chart showing the functional responsiveness in relation to IB4 staining. Note the virtual absence of menthol responses and the late onset of a large proportion of cinnamaldehyde-responsive neurons within the IB4⁺ population. D, Graph showing the amount of cinnamaldehyde-responsive cells in relation to IB4 staining, showing a significant increase of cinnamaldehyde sensitivity between P14 and adult in the IB4-positive population. CA, Cinnamaldehyde; Cap, capsaicin; Men, menthol; neg, negative; pos, positive.