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RT-PCR reveals a reduction in the top1 mRNA transcription level. Shown are the results of an RT-PCR analysis of RNA prepared from the heads of wild-type (WT) flies and top1JS mutant flies. Primers used in this assay amplified part of the first exon of the top1 gene. The internal control used in this assay is fly actin gene CG7478-PA (Act79B). The heads of top1JS mutants display a significant reduction (∼12.5-fold) in the level of top1 transcripts. Calibration: 10 mV, 200 ms.
