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. 2007 Jul 11;27(28):7508–7519. doi: 10.1523/JNEUROSCI.0705-07.2007

Figure 1.

Figure 1.

Expression of ephrinB3 in HEK293 cells clusters glutamate synaptic vesicles. A, The ephrinB3- or GFP-expressing HEK293 cells were cocultured with 10–12 DIV hippocampal neurons for 2 d. VGluT1 staining revealed formation of glutamatergic presynaptic terminals in axons crossing HEK293 cells expressing ephrinB3, but not those expressing GFP. Axons are highlighted by arrows in the differential interference contrast pictures. Scale bar, 10 μm. B, Number of VGluT1 puncta in axons crossing ephrinB3-expressing HEK293 cells was greater than in those that contact GFP-expressing HEK293 cells (ephrinB3, n = 17; GFP, n = 33; *p < 1 × 10−10). No significant increase in GAD65 clustering was induced by ephrinB3 (ephrinB3, n = 17; GFP, n = 22; p > 0.5). C, The VGluT1 interpuncta distance was significantly larger in axons contacting GFP-expressing HEK293 cells compared with ephrinB3-expressing cells (p < 0.05). D, E, Total area and integrated intensity of VGluT1 puncta for axons contacting ephrinB3-expressing HEK293 cells were greater than those crossing GFP-expressing cells (ephrinB3, n = 17; GFP, n = 33; *p < 1 × 10−6). No significant change in GAD65 signal was induced by ephrinB3 expression (ephrinB3, n = 17; GFP, n = 22; p > 0.5). All error bars indicate SEM.