Table 1.

Primers for TRPC mRNA

Gene and direction	Primer sequence	Base pairs	Product identitya or accession numberb
TRPC1 forward	ATGGGACAGATGTTACAA	400	NM_011643.1a
TRPC2 forward	CGCTGGGCACTCTGCAGA	370	U40981b
TRPC3 forward	AGGACATATTCAAGTTCA	340	NM_019510.1a
TRPC4 forward	CAGATATCTCTGGGAAGGATG	400	NM_016984b
TRPC5 forward	TTCTCTTTATCTACTGCC	360	NM_009428.1a
TRPC6 forward	TTCATGGTCATATTCATC	320	NM_013838.1a
All TRPC reverse	TGGAGCRAAYTTCCAYTC (R = G or A and Y = C or T)
GAPDH
Forward	ACCACAGTCCATGCCATCAC	450	NM_199472b
Reverse	TCCACCACCCTGTTGCTGTA

All primers are referenced to Tesfai et al. (2001).

^aIdentity numbers of product sequences for isoforms detected in cells, which were obtained from the NIH–NCBI website using the BLAST protocol.

^bAccession numbers for isoforms absent in cells, from which primers were designed.