Figure 4.

FRAP of RGC neurons expressing CFP-tau. a, Time-lapse fluorescence images of axon (t = 0 at end of bleaching). a, b, Red vertical lines indicate the bleached region. The boxed area (a) defines the region used for the kymograph (b). Scale bars, 10 μm. c, Normalized signal intensities along the axon before bleaching (black), directly after bleaching (red), and 194 s after bleaching (blue), showing ∼50% recovery. Red lines in center delineate the area for measuring the intensity recovery and hence the diffusion coefficient, shown in d as a percentage of initial level (half time of recovery, ∼180 s). e, Apparent diffusion coefficients of GFP, CFP-tau, and 3 CFP-tau mutants derived from FRAP.